Matrix-Assisted Pulsed-Laser Evaporation Direct-Write (MAPLE DW) has been emerging as a promising biological construct fabrication technique. Two MAPLE DW process challenges have been studied: 1) the effects of laser fluence and material properties of material being transferred on the droplet size in direct writing glycerol-water droplets; and 2) the effect of laser fluence on the post-transfer cell viability and the cell recovery ability of yeast cells. Some main conclusions are: 1) at a given glycerol concentration ratio, the droplet diameter is linearly dependent on the laser fluence; and the slope of this relationship is dependent on the glycerol concentration; 2) the droplet diameter has no systematic relationship with the glycerol concentration ratio; and 3) yeast cell viability decreases as the laser fluence increases and some of the MAPLE DW process-induced cell damage is reversible. Future work will focus on direct writing of mammalian cells.